Classification of Leukocytes Using Convolutional Networks

 

Mathematik | Informatik

 

Raphael Zumbrunn, 2002 | Adelboden, BE

 

Modern medicine relies heavily on the analysis of white blood cells to diagnose different types of disease. Most of the methods employed nowadays require large and expensive machinery and are therefore unavailable in large parts of the world. This paper suggests a machine-learning-based approach to classify leukocytes using only a phone, a cheap microscope, and a 3D-printed phone-holder. On validation, the model reached an accuracy of 85%. The accuracy under real-life conditions has not yet been evaluated.

Fragestellung

The aim of this project was to create a lightweight and portable solution to classify white blood cells in stained blood smears.

Methodik

In order to do this, a convolutional neural network was trained and ported to an Android app. To use the app with a microscope, a 3D-printable phone holder was used. The model was created using TensorFlow Lite and Keras. For the evaluation of the model, a small portion of the data was held back during training. To make the hyperparameter search easier, the Talos framework was used. To increase accuracy and reliability, multiple different networks with varying architecture were combined. The prediction is given by majority vote. This combined network has not yet been ported to Android.

Ergebnisse

A real-life-applicable app based on the image classification example provided by TensorFlow was created. The app contains a pretrained model. The model reaches 85% accuracy on validation. The accuracy under real-life conditions has not yet been evaluated but is estimated to be significantly lower. The app allows the user to zoom in and out to adjust to different hardware magnifications.

Diskussion

The main difficulties for this project were small datasets and low phone camera resolution. The small dataset led to a model that overfit very quickly and had to be stopped in order to remain generalizable. The low resolution was a problem, as the network had to work with fewer datapoints and more distortion when compared to the training images. The focus now is to improve accuracy under these suboptimal conditions. One way to do this would be with more preprocessing and data. A possible and very reasonable addition to the project would be to move from image classification to object recognition or segmentation. This would allow us to more easily automate the whole process of creating a blood count, because as of now, a person still has to focus the cells manually. This new architecture could be combined with a motorized microscopy table to make a fully automatic blood classifier.

Schlussfolgerungen

Ultimately, I managed to build a neural network that can accurately classify leukocytes during validation. The accuracy under real-life conditions remains to be evaluated. Because the phone adaptor is already done, and only minor additions must be made to the app, I now only have to focus on optimizing the network. If I manage to do this, this could be a great start for machine-learning-assisted biology education and remote medicine.

 

 

Würdigung durch den Experten

Dr. Peter Ashcroft

The work combines image recognition and software development to build an automated cell classifier. Raphael has assimilated a significant knowledge of machine learning throughout this project. With these skills he constructed and trained his own image classifying neural network from scratch. He was able to transfer this to an app to allow mobile users to classify cells in histopathology slides. The work is well written, and the potential applications in medicine and education are well described. Raphael made exceptional progress with the app, although the accuracy remains to be tested.

Prädikat:

gut

 

 

 

Gymnasium und HMS Thun-Schadau
Lehrer: Ivan Riesen